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李祥瑞

2014-10-1 20:05:36   来源:南京农业大学动物医学院   浏览:10
姓名

李祥瑞

性 别
出生日期

1958年12月

籍 贯

河南新乡

毕业院校

研究方向:
分子与免疫寄生虫学、兽医公共卫生和动物细胞因子的基因克隆与应用

主要通过分子生物学手段,从分子水平阐明寄生虫病,特别是人兽共患寄生虫病的免疫病理、免疫逃避和免疫保护机理,为寄生虫的免疫预防提供新的手段和制品。同时,通过对动物免疫相关细胞因子基因的克隆和体外表达,获得有生物学活性的重组细胞因子。根据不同动物细胞因子在不同动物疾病中的作用,有效利用细胞因子的免疫调节作用,提高疫苗的免疫效果,为动物疫病的免疫预防提供新的途径和手段。





个人简历:
博士,教授,博士生导师,国务院政府特殊津贴获得者,南京农业大学“133重点人才工程”优秀学术带头人。曾任动物医学院副院长、院长。现兼任全国兽医专业学位研究生教育指导委员会委员兼秘书长,农业部全国动物防疫专家委员会委员,农业部第六届兽药评审专家,农业部血吸虫病防治专家咨询委员会委员,中国畜牧兽医学会兽医寄生虫学分会副理事长,中国兽医协会常务理事,中国畜牧兽医学会兽医食品卫生分会常务理事,兽医公共卫生分会常务理事,中国动物学会寄生虫学分委员会常务理事,《亚洲兽医病例报告》、《中国兽医师》、《南京农业大学学报》(自然科学版)、《畜牧与兽医》、《寄生虫与医学昆虫学报》、《中国兽药杂志》、《中国动物传染病学报》、《动物医学进展》等期刊编委。曾兼任教育部高等学校动物医学类教学指导委员会委员(2003-2013),国家自然基金委员会第十二届、十三届专家评审组成员。
1981.12河南农学院本科毕业,获学士学位,1988南京农业大学兽医系获硕士学位,1993年北京农业大学兽医学院获博士学位。1993~1995年中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室从事博士后研究。1996,12~1997,12,韩国国立汉城大学生物系分子遗传实验室从事博士后研究。2002年7月~2003年1月,去美国麻省大学医学院分子遗传与微生物学系访问,从事基因芯片研究。先后赴英国、丹麦、蒙古等国访问交流。
先后主持国家攀登计划专题、,国家863项目、国家自然科学基金项目、公益性行业专项子课题等多项科研项目。发表相关研究论文180余篇,其中SCI论文40多篇。主编、副主编出版著作7部,参编专著、教材等10余本。获得国家发明专利授权6项,制定公布实施行业标准1项。主持的《兽医寄生虫学》课程获得国家精品课程和江苏省精品课程。先后指导毕业博士研究生21名,其中外国留学博士生2名,在读博士生12名,包括外国留学生3名。指导毕业硕士研究生66名,在读18名。是我校第一位获得博士学位外国留学生指导教师。







代表性论著:
1. 李祥瑞主编, 动物寄生虫病彩色图谱,第二版。 中国农业出版社,北京,2011
2. Zhao-Rong Lun, Xiao-Guang Chen, Li-Xin Lu, Xiang-Rui Li, and Ming-Quan Xie, Are Tritrichomonas foetus and Tritrichomonas suis synonym? TRENDS in Parasitology,2005, 21(3),122-125
3. Feng He, Xiangrui Li, Phyllis Spatrick, Ryan Casillo, Shuyun Dong, and Allan Jacobson 。Genome-Wide Analysis of mRNAs Regulated by the Nonsense-Mediated and 5′ to 3′ mRNA Decay Pathways in Yeast. Molecular Cell, 2003,12(6),1439-1452
4. Charles I. Muleke, Yan Ruofeng, Xu Lixin, Sun Yanming, Li Xiangrui, Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus. J Vet Sci. 2006,7(3),249-255
5. Chunhua Li, Xiaofeng Wei, Lixin Xu, Xiangrui Li, Recombinant galectins of male and female Haemonchus contortus do not hemagglutinate erythrocytes of their natural host. Veterinary Parasitology, 2007, 144:299-303
6. S. YANMING, Y. RUOFENG, C. I. MULEKE, Z. GUANGWEI, X. LIXIN & L. XIANGRUI.*Vaccination of goats with recombinant galectin antigen induces partial protection against Haemonchus contortus infection. Parasite Immunology,2007,29,319-326
7. Charles I. Muleke Yan Ruofeng Xu Lixin; Bo Xinwen; Li Xiangrui. Cloning and sequence analysis of Hemonchus Contortus HC58cDNA. DNA Sequence, 2007,18(3), 176 - 183
8. Jing Wang, Ruofeng Yan, Lixin Xu and Xiangrui Li. The second glutamic acid in the C-terminal CRD affects the carbohydrate-binding properties of recombinant galectins of Haemonchus contortus. Veterinary Parasitology, 2007, 148 (3-4), 247-255
9. Charles I. Muleke, Yan Ruofeng, Sun Yanming, Zhao Guangwei, Xu lixin, Li Xiangrui. Vaccination of goats against Haemonchus contortus with a recombinant cysteine Protease. Small Ruminant Research, 2007,73, 95-102
10. Yanming Sun, Ruofeng Yan, Charles I. Muleke, Guangwei Zhao, Lixin Xu, Xiangrui Li. Recombinant galectins of Haemonchus contortus Parasite Induces Apoptosis in the Peripheral Blood Lymphocytes of Goat. International Journal of Peptide Research and Therapeutics. 2007,13(3),387-392
11. Qianming Xu Xiaokai Song Lixin Xu Ruofeng Yan Mohammed Ali A. Shah Xiangrui Li.Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis. Veterinary Parasitology,2008,156,319-323
12. Xiaokai Song, Lixin Xu, Ruofeng Yan, Xinmei Huang, Mohammed Ali A. Shah, Xiangrui Li. The optimal immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella, its cross immunity to Eimeria necatrix and Eimeria acervulina. Veterinary parasitology, 2009,159(1),30-36.
13. Liheng Liu, Lixin Xu, Fengbin Yan, Ruofeng Yan, Xiaokai Song, Xiangrui Li . Immunoproteomic analysis of the second-generation merozoite proteins of Eimeria tenella. Veterinary Parasitology, 2009, 164(2-4),173-182
14. Mohammad AliA. Shah, Roufeng Yan, Lixin Xu, Xiaokai Song,Xiangrui Li *. A recombinant DNA vaccine encoding Eimeria acervulina cSZ-2 induces immunity against experimental E.tenella infection. Veterinary Parasitology. 2010, 169,185–189
15. Shah Mohammed Ali A, Lixin Xu, Roufeng yan, Xiaokai Song, Xiangrui Li*. Cross immunity of DNA vaccine pVAX1-cSZ2-IL-2 to Eimeria tenella, E. necatrix and E. maxima. Experimental parasitology, 2010,124,330-333
16. Daosheng Yi, Lixin Xu, Ruofeng Yan, Xiangrui Li*, Haemonchus contortus: Cloning and characterization of serpin. Experimental Parasitology, 2010, 125, 363–370
17. Feng Bin Yan, Li Xin Xu, Li Heng Liu, Ruo Feng Yan, Xiao Kai Song, Xiang Rui Li * Immunoproteomic analysis of whole proteins from male and female adult Haemonchus contortus. The Veterinary Journal, 2010,18: 174-179
18. Hongyan Song, Ruofeng Yan, Lixin Xu, Xiaokai Song, Mohammed Ali A. Shah, Huili Zhu, Xiangrui Li*. Efficacy of DNA vaccines carrying Eimeria acervulina lactate dehydrogenase antigen gene against coccidiosis. Experimental Parasitology, 2010, 126, 224–231
19. Muhammad AliA. Shah & Xiaokai Song & Lixin Xu Roufeng Yan & HongyanS ong & Zhang Ruirui & Lei Chengyu & Xiangrui Li. TheDNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis. Parasitol Res, 2010, 107:747–750
20 Yang Hong, Hongxiao Han, Jinbiao Peng, Ye Li, Yaojun Shi, Zhiqiang Fu, Jinming Liu, Jiaojiao Lin, Xiangrui Li. Schistosoma japonicum: Cloning, expression and characterization of a gene encoding the a5-subunit of the proteasome. Experimental Parasitology, 2010, 126:517-525
21. Hongyan Song, Xiaokai Song, Lixin Xu, Ruofeng Yan, Muhammed Ali A Shah, Xiangrui Li*, Changes of cytokines and IgG antibody in chickens vaccinated with DNA vaccines encoding Eimeria acervulina lactate dehydrogenase. Vet Parasitol, 2010,173: 219-227
22. Muhammad Ali A. Shah, Xiaokai Song, Lixin Xu, Ruofeng Yan, Xiangrui Li *, Construction of DNA vaccines encoding Eimeria acervulina cSZ-2 with chicken IL-2 and IFN-c and their efficacy against poultry coccidiosis. Research in Veterinary Science, 2011, 90: 72–77
23. Geriletu, Lixin Xu, Xurihua, Xiangrui Li, Vaccination of chickens with DNA vaccine expressing Eimeria tenella MZ5-7 against coccidiosis. Veterinary Parasitology, 2011, 177: 6–12
24. Liu Wen-xing,1,2 Hu Sen,2 Qiao Zu-jian,2 Chen Wei-ye,2 Liu Lin-tao,2 Wang Fang-kun,2 Hua Rong-hong,2 Bu ZHi-gao,2∗ and Li Xiang-rui1∗, Expression, purification, and improved antigenic specificity of a truncated recombinant bp26 protein of Brucella melitensis M5-90: a potential antigen for differential serodiagnosis of brucellosis in ship and goats.Biotechnology and Applied Biochemistry, 2011,58(1): 32-38
25. K. Han, L. Xu, R. Yan, X. Song and X. Li*. Cloning, expression and characterization of NAD1-dependent glyceraldehyde-3-phosphate dehydrogenase of adult Haemonchus contortus. Journal of Helminthology, 2011, 85: 421–429
26. Wei Sun, Xiaokai Song, Ruofeng Yan, Lixin Xu, Xiangrui Li ∗. Vaccination of goats with a glutathione peroxidase DNA vaccine induced partial protection against Haemonchus contortus infection. Veterinary Parasitology, 2011, 182: 239-247
27. Yang Hong, Jinbiao Peng, Weibin Jiang, Zhiqiang Fu , Jinming Liu , Yaojun Shi, Xiangrui Li *, Jiaojiao Lin*. Proteomic analysis of Schistosoma japonicum schistosomulum proteins that are differentially expressed among hosts differing in their susceptibility to the infection. Molecular & Cellular Proteomics 10.8 10.1074/mcp.M110.006098–1
28. GuangWei Zhao, RuoFeng Yan, Charles I. Muleke YanMing Sun, LiXin Xu, XiangRui Li, Vaccination of goats with DNA vaccines encoding H11 and IL-2 induces partial protection against Haemonchus contortus infection. The Veterinary Journal, 2012, 191: 94–100
29. Kaikai Han, Lixin Xu, Ruofeng Yan, Xiaokai Song, Xiangrui Li. Molecular cloning, expression and characterization of enolase from adult Haemonchus contortus. Research in Veterinary Science, 2012, 92: 259–265
30. GuangWei Zhao, Bo Shen, Qing Xie, Li Xin Xu, Ruo Feng Yan, Xiao Kai Song, I.A. Hassan, Xiang Rui Li ∗Detection of Toxoplasma gondii in free-range chickens in China based on circulating antigens and antibodies. Veterinary Parasitology, 2012, 18, 572–77
31. Wei Sun, Xiaokai Song, Ruofeng Yan, Lixin Xu, Xiangrui Li. Cloning and characterization of a selenium-independent glutathione peroxidase (HC29) from adult Haemonchus contortus. J Vet Sci, 2012, 13(1), 49-58
32. Han K, Xu L, Yan R, Song X, Li X. Vaccination of goats with glyceraldehyde-3-phosphate dehydrogenase DNA vaccine induced partial protection against Haemonchus contortus. Vet Immunol Immunopathol. 2012, 149: 177-185
33. Zhu Z, Fu Z, Zhang M, Han Y, Hong Y, Li D, Zhao Z, Shi Y, Li X, Lin J. Protective efficacy evaluation induced by recombinant protein LHD-Sj23-GST of Schistosoma japonicum emulsified with three different adjuvants. Parasite Immunol.2012, 34(6):341-344.
34. Tao Lin • Xiangrui Li • Huochun Yao • Zuzhang Wei • Feifei Tan •Runxia Liu • Lichang Sun • Rong Zhang • Wenliang Li • Jiaqi Lu •Guangzhi Tong • Shishan Yuan. Use of reverse genetics to develop a novel marker porcine reproductive and respiratory syndrome virus. Virus Genes, 2012, 45:548–555
34. ZHAO Guang-wei, SHEN Bo, XIE Qing, XU Li-xin, YAN Ruo-feng, SONG Xiao-kai, Hassan Ibrahim Adam and LI Xiang-rui. Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China. Journal of Integrative Agriculture, 2012, 11(8): 1347-1353
36. Nanshan Qi , Mingfei Sun , Caiyan Wu , Shenquan Liao , Minna Lv , Xiangrui Liand Jianping Cai. Transcription Profiles of Eimeria tenella Apical Membrane Antigen Gene, Etama. Journal of Animal and Veterinary Advances 2012,11(9): 1312-1315
37. Huili Zhu, Ruofeng Yan, Song Wang, Xiaokai Song, Lixin Xu, Xiangrui Li. Identification and molecular characterization of a novel antigen of Eimeria acervulina. Molecular and Biochemical Parasitology, 2012, 186: 21-28
38. HUILI ZHU, LIXIN XU, RUOFENG YAN, XIAOKAI SONG, FANG TANG, SONG WANG and XIANGRUI LI. Identification and characterization of a cDNA clone-encoding antigen of Eimeria acervulina. Parasitology, 2012, 139: 1711–1719.
39.Tang F, Xu L, Yan R, Song X, Li X. A DNA vaccine co-expressing Trichinella spiralis MIF and MCD-1 with murine ubiquitin induces partial protective immunity in mice. J Helminthol. 2013, 87:24-33
40.Hongyan Song, Baofeng Qiu, Ruofeng Yan, Lixin Xu, Xiaokai Song, Xiangrui Li . The protective efficacy of chimeric SO7/IL-2 DNA vaccine against coccidiosis in chickens. Research in Veterinary Science. 2013, 94: 562–567.
41. Tao Lin, Xiangrui Li, Huochun Yao, Zuhang Wei, Runxia Liu, Yu Deng, Shaolun Zhai, Wenliang Li, Lichang Sun, Jinxue Long, Hongbiao Zhang, Jiaqi Lu and Shishan Yuan. Phylogenetic Diversity of Classical Swine Fever Virus(CSFV) Field Isolates from Outbreaks in China Between 2008 and 2011. Asian Journal of Animal and Veterinary Advances, 2013. DOI: 10.3923/ajava.2013
42.. Ruofeng Yan, Wei Sun, Xiaokai Song, Lixin Xu, Xiangrui Li ⇑Vaccination of goats with DNA vaccine encoding Dim-1 induced partial protection against Haemonchus contortus: A preliminary experimental study. Res. Vet. Sci. 2013, 95:189–199







科研项目:
已结题项目:
禽重要病毒的遗传变异分析 国家攀登计划
IBD-白细胞介素-2复合疫苗的研制 江苏省重点攻关项目A
白细胞介素-2增强机体免疫力机理研究 教育部回国人员科学基金A
畜禽新型高效免疫佐剂和疫苗的产业化 863计划子课题
重组牛、羊白细胞介素-2下游处理技术的研究和动物区域试验 海市科技兴农重点攻关项目
毛滴虫诊断规程 农业部行业标准
禽用抗球虫基因工程疫苗的研究 863计划
捻转血矛线虫半乳糖结合凝集素在免疫和疾病发生中的作用及机理研究 国家自然基金
捻转血矛线虫半胱氨蛋白酶新基因Hc58生物学特性研究 国家自然基金
鸡球虫猪囊虫病基因工程疫苗研究创制 863计划子课题
捻转血矛线虫排泄分泌抗原蛋白组研究 江苏省自然基金
堆型艾美耳球虫免疫功能基因组研究 国家自然基金
鸡球虫免疫调节型多价DNA疫苗的研制 江苏省科技支撑计划


在研项目:
日本血吸虫病、包虫病、弓形虫病、附红细胞体病等重要人畜共患寄生虫病的防控技术研究子课题
农业部行业专项
捻转血矛线虫重组galectin抑制山羊外周血淋巴细胞细胞因子转录的通路研究 国家自然基金
家畜口蹄疫新型疫苗研制与生产工艺创新子课题偶蹄动物用新型免疫佐剂创制与产业化
十二五“863”计划子项目
鸡艾美耳球虫侵入部位特异性关键分子研究 国家自然基金





荣誉及奖项:
国务院政府特殊津贴
南京农业大学优秀研究生导师
中国农业科学优秀审稿专家
全国兽医专业学位研究生教育先进工作者
南京农业大学“133重点人才工程”优秀学术带头人
江苏省优秀硕士学位论文指导教师




联系方式:
地 址: 南京市卫岗1号
邮 编: 210095
电 话: 025-84399000 025-84395904
传 真: 025-84399000
E-mail: lixiangrui@njau.edu.cn




 
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